期刊
BIOSENSORS & BIOELECTRONICS
卷 63, 期 -, 页码 425-431出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.07.078
关键词
Electrochemical; Signal amplification; Silver nanoparticles; Hybridization chain reaction
类别
资金
- National Natural Science Foundation of China [21264001, 21004009]
- Excellent Talents of Xuzhou Medical College [D2014007]
- Natural Science Foundation of Jiangxi Province [20114BAB213010]
- Young Scientist Foundation of Jiangxi Province [20133BCB23020]
A new strategy to combine Zn2+ assistant DNA recycling followed with hybridization chain reaction dual amplification was designed for highly sensitive electrochemical detection of target DNA. A gold electrode was used to immobilize molecular beacon (MB) as the recognition probe and perform the amplification procedure. In the presence of the target DNA, the hairpin probe 1 was opened, and the DNAzyme was liberated from the caged structure. The activated DNAzyme hybridized with the MB and catalyzed its cleavage in the presence of Zn2+ cofactor and resulting in a free DNAzyme strand. Finally, each target-induced activated DNAzyme underwent many cycles triggering the cleavage of MB, thus forming numerous MB fragments. The MB fragments triggered the HCR and formed a long double-helix DNA structure. Because both H1 and H2 were labeled by biotin, a lot of SA-ALP was captured on the electrode surface, thus catalyzing a silver deposition process for electrochemical stripping analysis. This novel cascade signal amplification strategy can detect target DNA down to the attomolar level with a dynamic range spanning 6 orders of magnitude. This highly sensitive and specific assay has a great potential to become a promising DNA quantification method in biomedical research and clinical diagnosis. (C) 2014 Elsevier B.V. All rights reserved.
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