期刊
PARASITOLOGY INTERNATIONAL
卷 73, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2019.101941
关键词
Malaria; PCR; Dried blood spots; Limit of detection; FTA Elute Card
类别
资金
- JSPS KAKENHI [17H04650, 19K07532]
- Ohyama Health Foundation Inc.
- Grants-in-Aid for Scientific Research [17H04650, 19K07532] Funding Source: KAKEN
Polymerase chain reaction (PCR) is an essential diagnostic method for highly sensitive detection of Plasmodium infected erythrocytes in patients with malaria. This study compared the performance of filter papers used for the preparation of dried blood spots (DBS) in detecting Plasmodium by PCR. Whole blood spiked with P. falciparum-infected erythrocytes to obtain samples with various levels of parasitemia were applied to Whatman 3MM Chr papers, FTA Cards, or FTA Elute Cards to prepare the DBS. DNA was purified from the DBS using a DNA purification kit and used as the template for nested PCR. In probit analysis, the estimated limit of detection (LoD) was 5.5 parasites/mu L blood for Whatman 3MM Chr papers and FTA Cards and 1.6 parasites/mu L blood for the FTA Elute Card. This result suggested that the DBS prepared on an FTA Elute Card yield the best template DNA for subsequent high-sensitivity PCR-based detection of P. falciparum-infected erythrocytes. This finding can help improve the accuracy of malarial diagnostic tests.
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