期刊
BIOSENSORS & BIOELECTRONICS
卷 65, 期 -, 页码 307-313出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.10.012
关键词
Poly(o-phenylenediamine)/Au nanocomposite; Poly(vinyl ferrocene-2-aminothiophenol)/Au nanocomposite; Electrochemical immunosensor; Carcinoembryonic antigen; Alpha-fetoprotein
类别
资金
- National Natural Science Foundation of China [21273153]
- Beijing Natural Science Foundation [2132008]
- Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality [IDHT20140512]
- Research Base Construction Projects of Beijing Municipal Education Commission
In this work, a simple and sensitive multiplexed immunoassay protocol for simultaneous electrochemical determination of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) was designed using redox-active nanocomposites. As the redox-active species, the poly(o-phenylenediamine) (POPD)/Au nanocomposite and poly(vinyl ferrocene-2-aminothiophenol) (poly(VFc-ATP))/Au nanocomposite were obtained by one-step method which HAuCl4 was used as the oxidant. With Au nanoparticles (AuNPs), the nanocomposites were successful to immobilize labeled anti-CEA and anti-AFP as the immunosensing probes. The proposed electrochemical immunoassay enabled the simultaneous monitoring of AFP and CEA in a wide range of 0.01-100 ng mL(-1). The detection limits was 0.006 ng mL(-1) for CEA and 0.003 ng mL(-1) for AFP (S/N=3). The assay results of serum samples with the proposed method were well consistent with the reference values from standard ELISA method. And the negligible cross-reactivity between the two analytes makes it possesses potential promise in clinical diagnosis. (C) 2014 Elsevier B.V. All rights reserved.
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