期刊
NUCLEIC ACIDS RESEARCH
卷 48, 期 6, 页码 3211-3227出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz1239
关键词
-
资金
- Marie Curie Initial Training Network [607720]
- Ministry of Education, Youth and Sports (MEYS) National Sustainability Programme (NPU) project [NPU I LO1419]
- Charles University
- Czech Science Foundation [GACR 18-19395S]
- MEYS [NPU I LO1609]
- European Structural and Investment Funds grant for the Croatian National Centre of Research Excellence in Personalized Healthcare [KK.01.1.1.01.0010]
- Croatian National Centre of Research Excellence for Data Science and Advanced Cooperative Systems [KK.01.1.1.01.0009]
- Croatian Science Foundation [IP-2014-09-6400]
- Ministry of Education, Youth and Sports (MEYS) [LM2011032, LM2015040]
- BIOCEV European Regional Development Fund [CZ.1.05/1.1.00/02.0109]
- MEYS (Czech-BioImaging large research infrastructure project) [LM2015062 [CZ.02.1.01/0.0/0.0/16 013/0001775]]
- Biomodels for Health -Centre for Model Organisms [LO1419, CZ.2.16/3.1.00/21547]
- CESNET [LM2015042]
- Ministry of Education, Youth, and Sports (MEYS) National Sustainability Programme (NPU) project [NPU I LO1419]
Tens of thousands of rapidly evolving long non-coding RNA (lncRNA) genes have been identified, but functions were assigned to relatively few of them. The lncRNA contribution to the mouse oocyte physiology remains unknown. We report the evolutionary history and functional analysis of Sirena1, the most expressed lncRNA and the 10th most abundant poly(A) transcript in mouse oocytes. Sirena1 appeared in the common ancestor of mouse and rat and became engaged in two different post-transcriptional regulations. First, antisense oriented Bob pseudogene insertion into Sirena1 exon 1 is a source of small RNAs targeting Sob mRNA via RNA interference. Second, Sirena1 evolved functional cytoplasmic polyadenylation elements, an unexpected feature borrowed from translation control of specific maternal mRNAs. Sirena1 knock-out does not affect fertility, but causes minor dysregulation of the maternal transcriptome. This includes increased levels of Elob and mitochondrial mRNAs. Mitochondria in Sirena1(-/-) oocytes disperse from the perinuclear compartment, but do not change in number or ultrastructure. Taken together, Sirenal contributes to RNA interference and mitochondrial aggregation in mouse oocytes. Sirena1 exemplifies how lncRNAs stochastically engage or even repurpose molecular mechanisms during evolution. Simultaneously, Sirenai1 expression levels and unique functional features contrast with the lack of functional importance assessed under laboratory conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据