期刊
BIOSENSORS & BIOELECTRONICS
卷 72, 期 -, 页码 25-30出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.05.001
关键词
Liquid crystals; Sensor; Cationic surfactant; Acetylcholinesterase; Myristoylcholine; Inhibitor
类别
资金
- National Natural Science Foundation of China [21373128]
- Scientific and Technological Projects of Shandong Province of China [2014GSF117001]
- Natural Science Foundation of Shandong Province of China [ZR2011BM017]
- Project of Sinopec [P13045]
In this paper, construction of the liquid crystal (LC)-based sensing platform for simple and sensitive detection of acetylcholinesterase (AChE) and its inhibitor using a cationic surfactant-decorated LC interface was demonstrated. A change of the optical images of LCs from bright to dark appearance was observed when the cationic surfactant, myristoylcholine chloride (Myr), was transferred onto the aqueous/LC interface, due to the formation of a stable surfactant monolayer at the interface. A dark-to-bright change of the optical appearance was then observed when AChE was transferred onto the Myr-decorated LC interface. The sensitivity of this new type of LC-based sensor is 3 orders of magnitude higher in the serum albumin solution than that only in the buffer solution. Noteworthy is that the AChE LC sensor shows a very high sensitivity for the detection of the enzyme inhibitor, which is around 1 fM. The constructed low-cost LC-based sensor is quite simple and convenient, showing high promise for label-free detection of AChE and its inhibitors. (C) 2015 Elsevier B.V. All rights reserved.
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