期刊
BIOSENSORS & BIOELECTRONICS
卷 70, 期 -, 页码 411-417出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.03.046
关键词
Graphene-polyaniline nanocomposite; Glucose oxidase; Biosensor; Selectivity
类别
资金
- Funds of Key Laboratory of Marine Ecosystem and Biogeochemistry of SOA [LMEB201403]
- NSF of China [91128212]
- Public Science and Technology Research Program Foundation of Ocean [201405036-3]
A biosensor was fabricated by immobilizing glucose oxidase (GOD) into nanostructured graphene (GRA)conducting polyaniline (PANI) nanocomposite, which was based on electrochemical polymerization of aniline in GRA synthesized by using electrochemical expansion of graphite in propylene carbonate electrolyte. Scanning electron spectroscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the morphology and performance of the as-prepared biosensor, respectively. Amperometric measurements were carried out to optimize test conditions (pH and applied potential) of the biosensor. Under the optimal conditions, the biosensor showed a linear range from 10.0 mu M to 1.48 mM (R-2=0.9988) with a sensitivity of 22.1 mu A mM(-1) cm(-2), and a detection limit of 2.769 mu M (S/N=3). The apparent Michaelis-Menten constant (K-M(a)) was estimated to be 3.26 mM. The interference from glycine (Gly), D-galactose (D-Gal), urea (Urea), L-phenylalanine (L-Phe), ascorbic acid (AA), and L-tyrosine (L-Tyr) was also investigated. The results indicated that the biosensor exhibit high sensitivity and superior selectivity, providing a hopeful candidate for glucose biosensing. (C) 2015 Elsevier B.V. All rights reserved.
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