期刊
NEW PHYTOLOGIST
卷 229, 期 1, 页码 126-139出版社
WILEY
DOI: 10.1111/nph.16477
关键词
flowering time; hypoxia; N-degron pathway; polycomb; PRC2; proteolysis; root; vernalization
资金
- European Research Council Starter Grant [ERC-STG 715441-GasPlaNt]
- Biotechnology and Biological Sciences Research Council [BB/M020568/1, BB/M007820/1, BB/M029441/1]
- EMBO Long Term Fellowship [ALTF 219-2018]
- BBSRC [BB/M020568/1] Funding Source: UKRI
VRN2, a subunit of PRC2, is regulated by O-2 and the N-degron pathway, showing differential activity in plant development. Its stability affects flowering and root architecture, with environmental cues influencing its accumulation and function.
VERNALIZATION2 (VRN2), an angiosperm-specific subunit of the polycomb repressive complex 2 (PRC2), is an oxygen (O-2)-regulated target of the PCO branch of the PRT6 N-degron pathway of ubiquitin-mediated proteolysis. How this post-translational regulation coordinates VRN2 activity remains to be fully established. Here we useArabidopsis thalianaecotypes, mutants and transgenic lines to determine how control of VRN2 stability contributes to its functions during plant development. VRN2 localizes to endogenous hypoxic regions in aerial and root tissues. In the shoot apex, VRN2 differentially modulates flowering time dependent on photoperiod, whilst its presence in lateral root primordia and the root apical meristem negatively regulates root system architecture. Ectopic accumulation of VRN2 does not enhance its effects on flowering, but does potentiate its repressive effects on root growth. In late-flowering vernalization-dependent ecotypes, VRN2 is only active outside meristems when its proteolysis is inhibited in response to cold exposure, as its function requires concomitant cold-triggered increases in other PRC2 subunits and cofactors. We conclude that the O-2-sensitive N-degron of VRN2 has a dual function, confining VRN2 to meristems and primordia, where it has specific developmental roles, whilst also permitting broad accumulation outside of meristems in response to environmental cues, leading to other functions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据