期刊
NEW PHYTOLOGIST
卷 226, 期 2, 页码 541-554出版社
WILEY
DOI: 10.1111/nph.16386
关键词
evolution; intracellular infection; NF-YA1; NODULE INCEPTION (NIN); nodulation; Parasponia; rhizobium
资金
- NWO-VICI grant [865.13.001]
- ENSA project - Bill & Melinda Gates Foundation
- CSC Scholarship [201303250067]
- Ministry of Research, Technology and Higher Education of the Republic of Indonesia (RISET-PRO grant) [8245-ID]
Nitrogen-fixing nodulation occurs in 10 taxonomic lineages, with either rhizobia or Frankia bacteria. To establish such an endosymbiosis, two processes are essential: nodule organogenesis and intracellular bacterial infection. In the legume-rhizobium endosymbiosis, both processes are guarded by the transcription factor NODULE INCEPTION (NIN) and its downstream target genes of the NUCLEAR FACTOR Y (NF-Y) complex. It is hypothesized that nodulation has a single evolutionary origin c. 110 Ma, followed by many independent losses. Despite a significant body of knowledge of the legume-rhizobium symbiosis, it remains elusive which signalling modules are shared between nodulating species in different taxonomic clades. We used Parasponia andersonii to investigate the role of NIN and NF-YA genes in rhizobium nodulation in a nonlegume system. Consistent with legumes, P. andersonii PanNIN and PanNF-YA1 are coexpressed in nodules. By analyzing single, double and higher-order CRISPR-Cas9 knockout mutants, we show that nodule organogenesis and early symbiotic expression of PanNF-YA1 are PanNIN-dependent and that PanNF-YA1 is specifically required for intracellular rhizobium infection. This demonstrates that NIN and NF-YA1 have conserved symbiotic functions. As Parasponia and legumes diverged soon after the birth of the nodulation trait, we argue that NIN and NF-YA1 represent core transcriptional regulators in this symbiosis. BLACK CIRCLEBLACK CIRCLEBLACK CIRCLEBLACK CIRCLE
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