期刊
BIOSENSORS & BIOELECTRONICS
卷 74, 期 -, 页码 652-659出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.07.008
关键词
Foodborne bacteria; Simultaneous detection; Triple-tagging multiplex PCR; Electrochemical genosensing; Magneto-actuated electrode
类别
资金
- BioMaX: Novel diagnostic bio-assays based on magnetic particles Marie Curie Initial Training Networks (ITN) Call: FP7-PEOPLE-ITN
- Ministry of Science and Innovation (MEC), Madrid [BIO2013-41242-R]
- General-itat de Catalunya [2014SGR572]
- European Commission [317420]
Simultaneous detection of Salmonella enterica, Listeria monocytogenes and Escherichia coli based on triple-tagging multiplex PCR and electrochemical magneto genosensing on silica magnetic particles is reported. A set of tagging primers were selected for the specific amplification of yfiR (375 bp), hlyA (234 bp) and eaeA (151 bp), being one of the primers for each set labelled with fluorescein, biotin and digoxigenin coding for S. enterica, L. monocytogenes and E. coli, respectively. Afterwards, electrochemical magneto genosensing of the bacteria was achieved by using silica magnetic particles as a carrier and three different electrochemical reporters, specific for each pathogen. This method was able to clearly distinguish among the pathogenic bacteria tested within 50 min, with detection limits ranging from 12 to 46 pg mu L-1. (C) 2015 Elsevier B.V. All rights reserved.
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