期刊
BIOSENSORS & BIOELECTRONICS
卷 67, 期 -, 页码 146-153出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.07.080
关键词
Human iPSC-CM-based biosensor array; Impedance detection technology; Beating pattern function; hERG inhibitor recognition; Drug cardiotoxicity assessment
类别
资金
- Major International Cooperation Project of Natural Science Foundation of China [61320106002]
- Marine Public Welfare Project of China [201305010]
- National Natural Science Foundation of China [31228008]
High-throughput and high clinical relevance methods are demanded to predict the drug-induced cardiotoxicity in pharmaceutical and biotechnology industries to effectively decrease late-stage drug attrition. In this study, human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) were integrated into an interdigital impedance sensor array to fabricate a high performance iPSC-CM-based biosensor array with high-throughput and high-consistency beating pattern. Typical withdrawal approved drugs (astemizole, sertindole, cisapride, and droperidol) with hERG inhibition and positive control E-4031 were employed to determine the beating pattern function. From the results, it can be concluded that this iPSC-CM-based biosensor array can specifically differentiate the hERG inhibitors from the non-hERG inhibition compounds through beating pattern function. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据