期刊
NATURE METHODS
卷 17, 期 3, 页码 343-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41592-020-0759-2
关键词
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资金
- Novartis Research Foundation
- Novartis Institutes for Biomedical Research Presidential Postdoctoral Fellowship
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program [742576]
- Swiss National Science Foundation [310030B_152833/1, 135196/1]
- Boehringer Ingelheim Fonds
- NBRP from AMED
- NBRP/Fundamental Technologies Upgrading Program from AMED
- European Research Council (ERC) [742576] Funding Source: European Research Council (ERC)
- Swiss National Science Foundation (SNF) [310030B_152833] Funding Source: Swiss National Science Foundation (SNF)
Virtual realities are powerful tools to analyze and manipulate interactions between animals and their environment and to enable measurements of neuronal activity during behavior. In many species, however, optical access to the brain and/or the behavioral repertoire are limited. We developed a high-resolution virtual reality for head-restrained adult zebrafish, which exhibit cognitive behaviors not shown by larvae. We noninvasively measured activity throughout the dorsal telencephalon by multiphoton calcium imaging. Fish in the virtual reality showed regular swimming patterns and were attracted to animations of conspecifics. Manipulations of visuo-motor feedback revealed neurons that responded selectively to the mismatch between the expected and the actual visual consequences of motor output. Such error signals were prominent in multiple telencephalic areas, consistent with models of predictive processing. A virtual reality system for adult zebrafish therefore provides opportunities to analyze neuronal processing mechanisms underlying higher brain functions including decision making, associative learning, and social interactions. Complex behaviors and the underlying neural activity in adult zebrafish can be accessed through a virtual reality system in combination with two-photon microscopy.
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