期刊
NATURAL PRODUCT RESEARCH
卷 35, 期 22, 页码 4703-4708出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2019.1710701
关键词
Glycosides; DNA polymerase; Cancer cell lines; Antitumoral agents; Natural Products
资金
- UNSL [PROICO 02-2516]
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 2017-0785]
- CONICET [PIP 090-2015]
- Alexander von Humboldt Foundation
A phytochemical study was conducted on three native plant species from central-western Argentina, revealing potential anti-tumor activities in certain compounds. Verbascoside showed strong inhibitory effects on DNA polymerase and cytotoxicity against human melanoma and colorectal cell lines, indicating its potential as a therapeutic agent.
A phytochemical study was performed on three native plant species from the central-western zone of Argentina: Buddleja cordobensis Grisebach, Baccharis salicina Torr. & A. Gray and Nepeta cataria L. We could obtain verbascoside (1) from B. cordobensis. From N. cataria, we could obtain 1, 5, 9-epi-deoxyloganic acid (2) L. Finally, we could isolate 2-beta-(L-rhamnopyranosyl)-3-angeloyloxy-15-acetyloxy-7,13(14)-E-dien-ent-labdane (3) and 2-beta-(L-rhamnopyranosyl)-3-alpha-angeloyloxy-15-hydroxy-7,13(14)-E-dien-ent-labdane (4) from B. salicina. Moreover, three derivatives from 1, and one semi-synthetic derivative from 2, were prepared. PCR reaction was used to analyse the activity against DNA polymerase and cell culture to determine cytotoxicity and antitumoral activity. Verbascoside (1) was strongly active in the nanomolar scale (IC50 = 356 nM) against DNA polymerization. Moreover, verbascoside was also strongly active in the nanomolar scale against human melanoma cell line (IC50 = 256 nM) and human colorectal cell line (IC50 = 320 nM). Furthermore, derivatives 6 and 7 were cytotoxic against both cancer cell lines.
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