期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 24, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.nano.2019.102109
关键词
Extracellular vesicles; Exosomes; Raman spectroscopy; Single particle analysis; Nanomedicine; Nanoparticles
资金
- Netherlands Organization for Scientific Research-Domain Applied and Engineering Sciences (NWO-TTW) [14193]
- VENI program of the Netherlands Organization for Scientific Research-Domain Applied and Engineering Sciences (NWO-TTW) [15924]
Extracellular Vesicles (EVs) can be used as biomarkers in diseases like cancer, as their lineage of origin and molecular composition depend on the presence of cancer cells. Recognition of tumor-derived EVs (tdEVs) from other particles and EVs in body fluids requires characterization of single EVs to exploit their biomarker potential. We present here a new method based on synchronized Rayleigh and Raman light scattering from a single laser beam, which optically traps single EVs. Rapidly measured sequences of the Rayleigh scattering amplitude show precisely when an individual EV is trapped and the synchronously acquired Raman spectrum labels every time interval with chemical information. Raman spectra of many single EVs can thus be acquired with great fidelity in an automated manner by blocking the laser beam at regular time intervals. This new method enables single EV characterization from fluids at the single particle level. (C) 2019 The Author(s). Published by Elsevier Inc.
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