期刊
NANOMEDICINE
卷 15, 期 2, 页码 163-181出版社
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2019-0363
关键词
cardiomyocytes; hypoxia; reperfusion injury; miRNA; myocardial infarction; PAMAM dendrimers; RNA interference
资金
- NANOMISSION, Department of Science and Technology
- New Delhi, India [SR/NM/NS-1154/2015]
Aim: Myocardial infarction is a tissue injury that leads to apoptosis of cardiomyocytes. This can be prevented by using miRNAs, but its delivery to cardiomyocytes is a major hurdle. We aimed to deliver miRNAs using poly(amidoamine)-histidine (PAMAM-His) nanocarriers to prevent apoptosis. Materials & methods: The PAMAM-His nanoparticles were synthesized and assessed for their transfection efficiency of miRNAs to prevent apoptosis in hypoxia/reperfusion-induced H9c2 as well as primary cultured cardiomyocytes. Results & conclusion: miRNAs-nanoparticle complexes exerted a significant antiapoptotic effect on the H9c2 and primary rat ventricular cardiomyocytes. Enhanced expression of antiapoptotic genes and decreased expression of proapoptotic genes were observed. PAMAM-His nanoparticles effectively delivered miRNAs to the cardiomyocytes and prevented the hypoxia/reperfusion-induced apoptosis critical in myocardial infarctions.
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