期刊
BIOSCIENCE REPORTS
卷 35, 期 -, 页码 -出版社
PORTLAND PRESS LTD
DOI: 10.1042/BSR20140126
关键词
chromatin looping; enhancer RNAs (eRNAs); mouse beta-globin locus; transcriptional activation
资金
- Pusan National University
- National Research Foundation of Korea [21A20131212499] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Enhancers are closely positioned with actively transcribed target genes by chromatin looping. Non-coding RNAs are often transcribed on active enhancers, referred to as eRNAs (enhancer RNAs). To explore the kinetics of enhancer-promoter looping and eRNA transcription during transcriptional activation, we induced the beta-globin locus by chemical treatment and analysed cross-linking frequency between the beta-globin gene and locus control region (LCR) and the amount of eRNAs transcribed on the LCR in a time course manner. The cross-linking frequency was increased after chemical induction but before the transcriptional activation of gene in the beta-globin locus. Transcription of eRNAs was increased in concomitant with the increase in cross-linking frequency. These results show that chromatin looping and eRNA transcription precedes the transcriptional activation of gene. Concomitant occurrence of the two events suggests functional relationship between them.
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