期刊
MOLECULES
卷 25, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/molecules25030703
关键词
colon cancer; beta-catenin; Z-ajoene; phosphorylation; casein kinase 1 alpha
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2011-0030074, 2018R1D1A3B07050361]
- Sookmyung Women's University Specialization Program Funding [1-201809-6]
- National Research Foundation of Korea [2018R1D1A3B07050361] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Aberrant activation of a Wnt/beta-catenin pathway results in nuclear accumulation of beta-catenin in colon cancer. Inhibiting beta-catenin is one strategy for treating colon cancer. Here, we identified Z-ajoene, a sulfur containing compound isolated from crushed garlic, as an inhibitor of colon cancer cell growth. Z-Ajoene repressed beta-catenin response transcriptional activity, intracellular beta-catenin levels, and its representative target protein levels (c-Myc and cyclin D1) in SW480 colon cancer cells. To clarify the regulatory mechanism of decreased beta-catenin levels, we examined the effect of Z-ajoene on beta-catenin phosphorylation, which is involved in beta-catenin degradation. Z-Ajoene promoted the phosphorylation of beta-catenin at Ser45 in a casein kinase 1 alpha (CK1 alpha)-dependent manner, which is an essential step in beta-catenin degradation in the cytosol. These findings indicate that Z-ajoene from garlic may be a potential chemotherapeutic agent by modulating CK1 alpha activity and the Wnt/beta-catenin signaling pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据