期刊
MOLECULAR PLANT
卷 12, 期 12, 页码 1561-1576出版社
CELL PRESS
DOI: 10.1016/j.molp.2019.10.015
关键词
rice; arbuscular mycorrhizal symbiosis; Myc factors; Nod factors; LysM receptor-like kinases
资金
- NSFC [31730103, 31825003, 31522007]
- National Key Basic Research Programme in China [2015CB158300]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27040207]
- International Partnership Program of Chinese Academy of Sciences [153D31KYSB20160074]
- National Key Laboratory of Plant Molecular Genetics
Symbiotic microorganisms improve nutrient uptake by plants. To initiate mutualistic symbiosis with arbuscular mycorrhizal (AM) fungi, plants perceive Myc factors, including lipochitooligosaccharides (LCOs) and short-chain chitooligosaccharides (CO4/CO5), secreted by AM fungi. However, the molecular mechanism of Myc factor perception remains elusive. In this study, we identified a heteromer of LysM receptor-like kinases consisting of OsMYR1/OsLYK2 and OsCERK1 that mediates the perception of AM fungi in rice. CO4 directly binds to OsMYR1, promoting the dimerization and phosphorylation of this receptor complex. Compared with control plants, Osmyr1 and Oscerk1 mutant rice plants are less sensitive to Myc factors and show decreased AM colonization. We engineered transgenic rice by expressing chimeric receptors that respectively replaced the ectodomains of OsMYR1 and OsCERK1 with those from the homologous Nod factor receptors MtNFP and MtLYK3 of Medicago truncatula. Transgenic plants displayed increased calcium oscillations in response to Nod factors compared with control rice. Our study provides significant mechanistic insights into AM symbiotic signal perception in rice. Expression of chimeric Nod/Myc receptors achieves a potentially important step toward generating cereals that host nitrogen-fixing bacteria.
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