期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 64, 期 2, 页码 -出版社
WILEY
DOI: 10.1002/mnfr.201901012
关键词
gut microbiota; inflammation; oxidative stress; oxidized pork; protein oxidation
资金
- National Natural Science Foundation of China [31901679]
- Postgraduate Research and Practice Innovation program of Jiangsu Province [KYCX18_1781]
- National Natural Science Foundation of Jiangsu Province, China [BK20190594]
Scope Reduced digestibility of foods containing oxidized proteins and the subsequent excessive accumulation of undigested components in the colon may cause changes in the intestinal flora composition. This study evaluates the characteristics of this change and the potential adverse effects on organisms. Methods and results Pork is cooked using sous-vide or at high temperature and pressure (HTP), then freeze-dried, resulting in different levels of oxidized damage. Mice are fed diets containing low- (LOP), medium- (MOP), or high-oxidative damage pork (HOP) for 12 weeks. HOP intake increases mice body weight, induces inflammatory response, and causes oxidative stress, as indicated by the accumulation of oxidative products. Increased serum LPS levels and downregulation of tight junction-related genes in the mucosa suggest mucosal barrier damage. Alterations in the cecal microbiota include reduced relative abundance of the mucin-degrading bacteria Akkermansia, beneficial bacteria Lactobacillus and Bifidobacterium, and H2S-producing bacteria Desulfovibrio and increased relative abundance of the pro-inflammatory bacteria Escherichia-Shigella and pathobiont Mucispirillum. Conclusion HOP intake causes the accumulation of oxidative products, increases body weight, damages the intestinal barrier, and induces oxidative stress and inflammatory response, likely by altering gut microbiota through protein oxidation (POX).
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