期刊
MOLECULAR IMAGING AND BIOLOGY
卷 22, 期 4, 页码 958-968出版社
SPRINGER
DOI: 10.1007/s11307-020-01473-0
关键词
Tumor-associated macrophage (TAM); Breast cancer; Iron oxide nanoparticle; Magnetic resonance imaging (MRI); Magnetic particle imaging (MPI)
资金
- CIHR [PJF 367445] Funding Source: Medline
Purpose Magnetic particle imaging (MPI) is an emerging molecular imaging technique that directly detects iron nanoparticles distributed in living subjects. Compared with imaging iron with magnetic resonance imaging (MRI), MPI signal can be measured to determine iron content in specific regions. In this paper, the detection of iron-labeled macrophages associated with cancer by MRI and MPI was compared. Procedures Imaging was performed on 4T1 tumor-bearing mice 16-21 days post-cancer cell implantation, 24 h after intravenous injection of Ferucarbotran, a superparamagnetic iron oxide (SPIO) or Ferumoxytol, an ultra-small SPIO. Images of living mice were acquired on a 3T clinical MRI (General Electric,n = 6) or MPI (Magnetic Insight,n = 10) system. After imaging, tumors and lungs were removed, imaged by MPI and examined by histology. Results MRI signal voids were observed within all tumors.In vivo, MPI signals were observed in the tumors of 4 of 5 mice after the administration of each contrast agent and in all excised tumors. Signal voids visualized by MRI were more apparent in tumors of mice injected with Ferumoxytol than those that received Ferucarbotran; this was consistent with iron content measured by MPI. Signal voids relating to macrophage uptake of iron were not detected in lungs by MRI, since air also appears hypointense.In vivo, MPI could not differentiate between iron in the lungsvsthe high signal from iron in the liver. However, once the lungs were excised, MPI signal was detectable and quantifiable. Histologic examination confirmed iron within macrophages present in the tumors. Conclusions MPI provides quantitative information onin vivoiron labeling of macrophages that is not attainable with MRI. The optimal iron nanoparticle for MPI in general is still under investigation; however, for MPI imaging of macrophages labeledin vivoby intravenous administration, Ferumoxytol nanoparticles were superior to Ferucarbotran.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据