期刊
MOLECULAR IMAGING AND BIOLOGY
卷 22, 期 4, 页码 873-882出版社
SPRINGER
DOI: 10.1007/s11307-019-01450-2
关键词
Oxidative stress imaging; Cystine transporter; FASu; Fluoroaminosuberic acid; Chirality; Isomers; Cancer PET imaging; Prostate cancer; Glioblastoma; TNBC
资金
- CIHR [201403COP, 329895]
- NSERC CREATE IsoSiM fellowship [448110]
- BC Leading Edge Endowment Fund
- National Research Council of Canada
Purpose The cystine transporter, system x(C)(-), plays a crucial role in sustaining redox homeostasis and is reported to be overexpressed in several cancer subtypes. 5-[F-18]Fluoroaminosuberic acid ([F-18]FASu) is a novel positron emission tomography (PET) tracer, which exhibits specific uptake via system x(C)(-). [F-18]FASu synthesis by the commonly used Kryptofix 2.2.2/K2CO3-facilitated fluorination method results in four diastereomers, as a result of 2 chiral centers at positions 2- and 5- of the tracer. We recently reported the synthesis of the optically pure 2S-[F-18]FASu from chiral precursors. Our preliminary results indicated preferential uptake of the 2S-isomer by tumor cells compared to 2R-[F-18]FASu. Few studies have investigated the biodistribution of chiral F-18-labeled amino acids. The aim of this study was to evaluate the imaging utility and biodistribution of the 5-position diastereomers as well as the racemic (2S,5R/S-) mixture in three different tumor models. Procedures In vitro tracer uptake experiments and Western blotting were performed in breast cancer (MDA-MB-231), glioblastoma (U-87), and prostate (PC-3) cancer cell lines. PET imaging and biodistribution studies were conducted in xenograft-bearing immunocompromised Rag2M female mice. Results All three tracer conformations allowed for the visualization of tumor xenografts at 1 h (for U-87 and PC-3 tumors) or 2 h (in the case of MDA-MB-231 xenografts) post-injection, with the racemate (2S,5R/S-) displaying similar image contrast as compared to the 5- position diastereomers and the 2S,5S-[F-18]FASu conformation exhibiting relatively higher contrast for imaging U-87 and PC-3 xenografts. Tumor uptake of the isomers was blocked by an excess of the non-radioactive standard, aminosuberic acid (ASu), confirming target specificity. All three isomers were excreted via the renal pathway. Biodistribution analyses showed that PC-3 tumors had the highest tracer uptake, and the accumulation (%ID/g) of the 2S,5R/S-, 2S,5S-, and 2S,5R- isomers was 9.19 +/- 1.14, 8.00 +/- 1.41, and 7.16 +/- 2.13 at 1 h post-injection, respectively. This gave corresponding tumor-to-muscle ratios of 33.68 +/- 9.52, 31.42 +/- 4.54, and 25.33 +/- 4.97, respectively. Conclusion Our data suggest that pure 2S-[F-18]FASu can be used to noninvasively image system x(C)(-) in a variety of cancers, either as the racemic mixture (2S,5R/S-) or optically pure form. Furthermore, this work shows potential utility of [F-18]FASu for detection of glioblastoma and prostate cancer.
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