期刊
MICROCHIMICA ACTA
卷 187, 期 1, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3998-0
关键词
Metal-organic framework; ZIF-8; Pepsin; Capillary electrochromatography; Monolithic column; Enantioseparation; Basic drugs; Glycidyl methacrylate; Nanoparticles; Self assembly
资金
- Natural Science Foundation of Jiangsu Province [BK20141353]
Poly(glycidyl methacrylate)-co-(ethylene dimethacrylate) [poly(GMA-co-EDMA)] monoliths were used as a support to grow a zeolitic imidazolate framework-8 (ZIF-8) via layer-by-layer self-assembly. Pepsin, acting as as chiral selector, was covalently linked to the surface of the amino-modified ZIF-8 through the Schiff base method. The material was characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Fourier transform infrared spectroscopy and elemental analysis. The pepsin-ZIF-8-poly( GMA-co-EDMA) column was utilized to the enantioseparation of the racemic forms of hydroxychloroquine (HCQ), chloroquine (CHQ), hydroxyzine (HXY), nefopam (NEF), clenbuterol (CLE) and amlodipine (AML). In comparison with a pepsin-poly(GMA-co-EDMA) monolithic column (without self-assembled ZIF-8 nanoparticles), the resolution is strongly enhanced (HCQ: 0.34 -> 2.50; CHQ: 0.45 -> 1.97; HXY: 0.39 -> 1.43; NEF: 0.27 -> 0.81; CLE: 0 -> 0.81; AML: 0.16 -> 0.72). Effects of self-assembly layers of ZIF-8, pepsin concentration, buffer pH values and applied voltage were investigated with hydroxychloroquine as the model analyte. The reproducibility of run-to-run, day-to-day and column-tocolumn were explored, and found to be satisfactory.
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