期刊
MICROCHIMICA ACTA
卷 187, 期 1, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3921-8
关键词
Biosensor; Escherichia coli; Zinc oxide; Graphene nanoflakes; Electrophoretic deposition
资金
- CSIR, India
- Department of Science and Technology, New Delhi, India [DST/INSPIRE/04/2015/000932]
- Science AMP
- Engineering Research Board (Govt. of India) [SB/S9/YSCP/SERB-DF/2018]
Aminopropyltrimethoxysilane (APTMS)-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (cGNF) were used in a composite that was electrophoretically deposited on an indium tin oxide (ITO) coated glass substrate. The modified ITO electrodes were characterized using various microscopic and spectroscopic techniques which confirm the deposition of the APTMS-ZnO/c-GNF composite. The electrodes have been used for the covalent immobilization of an Escherichia coli O157:H7 (E. coli)-specific DNA prob. Impedimetric studies revealed that the gene sensor displays linear response in a wide range of target DNA concentration (10(-16) M to 10(-6) M) with a detection limit of 0.1 fM. The studies on the cross-reactivity to other water-borne pathogens show that the bioelectrode is highly specific.
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