期刊
MICROCHIMICA ACTA
卷 187, 期 1, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-4055-8
关键词
Array channel; Mercapto group; Nanoparticles; Chip-based MSPME; Arsenic species; Online analysis; Sequential elution; SCC-7 cell; GSH-1A human hair
资金
- National Nature Science Foundation of China [21775113, 21575107, 21575108, 21375097, 21175102]
- Science Fund for Creative Research Groups of NSFC [20921062]
- MOE of China
- Large-Scale Instrument and Equipment Sharing Foundation of Wuhan University [LF20191236]
A dual column packed with a magnetic metal-organic framework composite (MFC) and mercapto-functionalized MFC nano-particles (MFC-SH) in microfluidic chip channels is described for array chip-based magnetic solid phase microextraction of arsenic species including arsenite [As(III)], arsenate [As(V)], monomethylarsonous acid (MMA) and dimethylarsinic acid (DMA) in SCC-7 cells. At pH 6, the MFC-SH adsorbs As(III) while MFC quantitatively adsorbs As(V), DMA and MMA. The As(III) adsorbed on theMFC-SH can be desorbed with a 2% solution of thiourea in 0.5 M HNO3. The arsenic species MMA, DMA and As(V) are retained on theMFC but can be desorbed by ammonia. A sequential elution strategy was employed to elute MMA, DMA, As(V) and As(III) one by one for subsequent on-line determination by ICP-MS. The limits of detection are 4.8, 6.3, 3.8 and 7.1 ng L-1 for As(V), DMA, MMA and As(III), respectively. The enrichment factors are between 23 and 25, and the throughput is 7 samples per hour. The arsenic species in (spiked) SCC-7 cell samples were analyzed by the online system with adequate recoveries (89-110%).
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