期刊
MICROCHEMICAL JOURNAL
卷 156, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2020.104764
关键词
Triple-helix molecular beacon; G-wires; Resonance light scattering; Label-free
资金
- National Natural Science Foundation of China [21775132]
- National Natural Science Foundation of Hunan province [2018JJ2388]
- Hunan 2011 Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization
- project of innovation team of the ministry of education [IRT_17R90]
In this work, a label-free resonance light scattering (RLS) biosensor based on triple-helix molecular switch (THMS) and G-quadruplex nanowires (G-wires) for HAV DNA and microRNA-31 detection was developed. The target can combine with the loop sequences of triple-helix, and opened the triple-helix structure as well as liberated the G-rich sequences which were self-assembled into G-wires in the presence of K+ and Mg2+ causing an obvious enhancement of RLS intensity. Meanwhile, the RLS signal at 304 nm was linear with the logarithm of target concentration in the range of 50 pM to 300 nM, and the limit of detection is as low as 7.1 pM for HAV DNA and microRNA-31, respectively. Moreover, qualitative analysis of HAV DNA and microRNA-31 was successfully achieved in human serum samples and cancer cell lysates. The biosensor holds a great promise for universal RLS sensing platform for sensitive detection of various targets just by changing the sequence of the THMS in the loop and be a biosensing platform for early clinical diagnosis and biomedical research.
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