期刊
MICROBIAL PATHOGENESIS
卷 139, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2019.103905
关键词
Immunogenicity; PiCV; Recombinant baculovirus; Virus-like particles
资金
- National Key Research and Development Program of China [2017YFD0501804, 2016YFD0501003]
- Shandong Provincial Natural Science Foundation [ZR2019QC010, ZR2016CQ18]
- China Postdoctoral Science Foundation [2015M580592, 2016T90637]
- Fundamental Research Funds of Shandong University [2018JC050]
Pigeon circovirus (PiCV) is able to infect racing and meat pigeons of all ages and is a key factor that triggers young pigeon disease syndrome (YPDS). PiCV vaccine research has been impeded because PiCV cannot be grown or propagated in cell cultures. Virus-like particles (VLPs), which can be generated by a wide range of expression systems, have been shown to have outstanding immunogenicity and constitute promising vaccines against a wide range of pathogens. Cap protein, which contains neutralizing antibody epitopes, is the only capsid protein of PiCV. In this study, the baculovirus expression system was utilized to express the PiCV Cap protein, which was self-assembled into VLPs with a spherical morphology and diameters of 15-18 nm. Specific antibodies against the Cap protein were induced after BALB/c mice immunized intramuscularly (i.m.) with VLPs combined with adjuvant. Based on these findings, PiCV VLPs may be a promising candidate vaccine against PiCV.
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