期刊
MICROBIAL PATHOGENESIS
卷 137, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2019.103766
关键词
Novel goose astrovirus (N-GoAstV); TaqMan; qRT-PCR; Detection; Transmission
资金
- China Agricultural Research System [CARS-42]
- Natural Science Foundation of China [31602068]
- Fujian Academy of Agriculture Science Innovative Research Team Project [STIT2017-1-5, STIT2017-3-10]
- Fujian Public Welfare Project [2018R1023-5]
- Young Talent Program Project [YC2015-12]
Recently, a novel goose astrovirus (N-GoAstV) was discovered in China, with the transmission route of N-GoAstV unclear. In this study, we developed a TaqMan-based real-time RT-PCR (qRT-PCR) assay for the detection of N-GoAstV infection. After the optimization of the qRT-PCR assay conditions, the results demonstrated that the lower limit of detection for N-GoAstV was 33.4 copies/mu L. No cross-reactivity was observed with other goose-origin viruses. Intra-assay and inter-assay variability were <= 1.36% and 2.34%, respectively. N-GoAstV was detected in both field samples, embryos and newly hatched goslings by qRT-PCR assay, provided the view that N-GoAstV may be both horizontally and vertically transmitted. The established qRT-PCR method showed high specificity, sensitivity, and reproducibility, which can be used in future investigations on the pathogenesis and epidemiology of N-GoAstV.
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