期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 79, 期 9, 页码 1422-1429出版社
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2015.1032882
关键词
Oryza sativa; developing grain; aleurone layer; phloem; aquaporin
类别
资金
- Bio-oriented Technology Research Advancement Institute (Brain) of Japan [Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN)]
- NARO Gender Equality Program
During rice grain filling, grain moisture content and weight show dynamic changes. We focused on the expression of all 33 rice aquaporins in developing grains. Only two aquaporin genes, OsPIP2;1 and OsTIP3;1, were highly expressed in the period 10-25days after heading (DAH). High-temperature treatment from 7 to 21 DAH abolished the dynamic up-regulation of OsPIP2;1 in the period 15-20 DAH, whereas OsTIP3;1 expression was not affected. Immunohistochemical analysis revealed that OsPIP2;1 was present in the starchy endosperm, nucellar projection, nucellar epidermis, and dorsal vascular bundles, but not in the aleurone layer. OsTIP3;1 was present in the aleurone layer and starchy endosperm. Water transport activity of recombinant OsTIP3;1 was low, in contrast to the high activity of recombinant OsPIP2;1 we reported previously. Our data suggest that OsPIP2;1 and OsTIP3;1 have distinct roles in developing grains.
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