期刊
MARINE DRUGS
卷 18, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/md18030136
关键词
low-molecular-weight fucoidan; radiation-induced fibrosis; TGF-beta 1; collagen I; Smad pathway
资金
- Ministry of Science and Technology [MOST 107-2320-B-019-001-MY2]
- Lo-Hsu Medical Foundation, Lotung Poh-Ai Hospital [10,908, 1909]
Radiation-induced fibrosis (RIF) occurs after radiation therapy in normal tissues due to excessive production and deposition of extracellular matrix proteins and collagen, possibly resulting in organ function impairment. This study investigates the effects of low-molecular-weight fucoidan (LMF) on irradiated NIH3T3 cells. Specifically, we quantified cellular metabolic activity, fibrosis-related mRNA expression, transforming growth factor beta-1 (TGF-beta 1), and collagen-1 protein expression, and fibroblast contractility in response to LMF. LMF pre + post-treatment could more effectively increase cellular metabolic activity compared with LMF post-treatment. LMF pre + post-treatment inhibited TGF-beta 1 expression, which mediates negative activation of phosphorylated Smad3 (pSmad3) and Smad4 complex formation and suppresses downstream collagen I accumulation. In addition, LMF pre + post-treatment significantly reduced actin-stress fibers in irradiated NIH3T3 cells. LMF, a natural substance obtained from brown seaweed, may be a candidate agent for preventing or inhibiting RIF.
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