期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 117, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2019.108638
关键词
Fatty acid; Membrane fluidity; Transmission electron micrograph; Proteomics
资金
- National Natural Science Foundation of China [31571914, 31501551]
- Shanghai Engineering Research Center Construction Special from Shanghai Municipal Science and Technology Commission [16DZ2280300]
- Startup Foundation for Doctors of Shanghai Ocean University [A2-2006-00-200360]
Shewanella putrefaciens is an important spoilage microorganism in chilled Pacific white shrimp. In order to study the cold adaptation behavior of S. putrefaciens, the growth pattern, fatty acid composition, protein content, cell membrane microstructure and membrane fluidity of the isolated strain QY38 cultured at different temperatures (30 degrees C, 10 degrees C, and 4 degrees C) were studied comparatively. The lag phases of S. putrefaciens QY38 cultured at 30 degrees C, 10 degrees C, and 4 degrees C were 2.95 h, 15.42 h and 62.79 h, respectively. The saturated fatty acid content in the bacterial cell decreased with the decrease in incubation temperature, while the content of palmitoleic acid (C16:1), lauric acid (C12:0), and myristic acid (C14:0) increased. Then the cold adaptation process was studied, and membrane fluidity decreased after moving from 30 degrees C to 4 degrees C and increased after a period of adaptation. The differential proteins expressed during cold adaptation process showed that the fatty acid metabolism regulon (FadR) and 3-hydroxyacyl-ACP dehydratases (FabA) were up-regulated during cold acclimation, while 3-oxoacyl-ACP synthase (FabB) and desaturase (Des) were down-regulated. It was hypothesized that in S. putrefaciens QY38, the unsaturated fatty acid was biosynthesized through anaerobic metabolic pathway to recover the membrane fluidity to some extent.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据