期刊
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
卷 57, 期 -, 页码 21-27出版社
ELSEVIER GMBH
DOI: 10.1016/j.jtemb.2019.09.001
关键词
Manganese; Caenorhabditis elegans; Oxidative stress; DNA damage response; Poly(ADP-ribosyl)ation
资金
- German Research Foundation (DFG) [BO 4103/2-1, INST 38/537-1]
- DFG Research Unit TraceAge [FOR 2558]
- Konstanz Research School Chemical Biology (KoRS-CB) [GSC 218]
- NIH Office of Research Infrastructure Programs [P40 OD010440]
- National Institute of Environmental Health Sciences (NIEHS) [R01 ES10563R01 ES07331]
Background and aim: When exceeding the homeostatic range, manganese (Mn) might cause neurotoxicity, characteristic of the pathophysiology of several neurological diseases. Although the underlying mechanism of its neurotoxicity remains unclear, Mn-induced oxidative stress contributes to disease etiology. DNA damage caused by oxidative stress may further trigger dysregulation of DNA-damage-induced poly(ADP-ribosyl)ation (PARylation), which is of central importance especially for neuronal homeostasis. Accordingly, this study was designed to assess in the genetically traceable in vivo model Caenorhabditis elegans the role of PARylation as well as the consequences of loss of pme-1 or pme-2 (orthologues of PARP1 and PARP2) in Mn-induced toxicity. Methods: A specific and sensitive isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to quantify PARylation in worms. Next to monitoring the PAR level, pme-1 and pme2 gene expression as well as Mn-induced oxidative stress was studied in wildtype worms and the pme deletion mutants. Results and conclusion: While Mn failed to induce PARylation in wildtype worms, toxic doses of Mn led to PAR-induction in pme-1-deficient worms, due to an increased gene expression of pme-2 in the pme-1 deletion mutants. However, this effect could not be observed at sub-toxic Mn doses as well as upon longer incubation times. Regarding Mn-induced oxidative stress, the deletion mutants did not show hypersensitivity. Taken together, this study characterizes worms to model PAR inhibition and addresses the consequences for Mn-induced oxidative stress in genetically manipulated worms.
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