期刊
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
卷 100, 期 3, 页码 1204-1212出版社
WILEY
DOI: 10.1002/jsfa.10131
关键词
Hermetia illucens; degreasing solvent; protein quality; nutritional value evaluation; in vitro digestibility; thermal properties
资金
- National Science Foundation of China [31701604]
- Key Programme of Hubei Provincial Department of Education [D20171501]
BACKGROUND The aim of this study was to select appropriate low-toxicity degreasing solvents to degrease black soldier fly (BSF, Hermetia illucens L.) larvae to prepare high-quality protein. Aqueous ethyl acetate was chosen as the solvent to extract BSF protein, and traditional solvents, such as petroleum ether, n-hexane, and isopropanol, were chosen as controls. RESULTS The meal degreased by aqueous ethyl acetate (the volume ratio of ethyl acetate to water is 90 to 10, EA + W10) shows a high degreasing rate (29.04%), crude protein content (562.3 g kg(-1)), essential amino acid index (EAAI, 95.57), and digestible indispensable amino acid score (DIAAS, 85). The digestibility of the degreased meal samples in the simulated in vitro intestine can reach 76.52%. Thermodynamic analysis and the apparent morphology of the protein fragments showed that the meal degreased by EA + W10 exhibited thermodynamic stability, which suggests that using aqueous ethyl acetate as the degreasing solvent did not affect the nutritional value of the degreased meal. CONCLUSION The results suggest that aqueous ethyl acetate (EA + W10) can be used as a novel solvent in the degreasing of BSF larvae meal to prepare high-quality protein with high EAAI and DIAAS and good digestibility. (c) 2019 Society of Chemical Industry
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