DPPH Radical-Scavenging Activity and Kinetics of Antioxidant Agent Hesperidin in Pure Aqueous Micellar Solutions

The antioxidant ability of bioactive agent hesperidin was assessed in terms of radical-scavenging activity (RSA) against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical in aqueous colloidal media containing micelle using UV-vis spectrophotometry. The DPPH assay was carried out at 25.0 +/- 0.1 degrees C and cationic surfactant CTAB and anionic surfactant SDS at various concentrations above the critical micelle concentration (CMC). The rates of the antioxidant reaction (R-s) of hesperidin were also measured in the micelle systems. The activity and rate of the DPPH radical scavenging by hesperidin were found to depend on concentration and nature of the surfactants used, so that both RSA and R-s values increase with increasing concentration of micelles CTAB and SDS. Finally, the micelle effects on the antioxidant efficiency were explained based on possible interaction modes between hesperidin and the micellar surfaces.