期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 142, 期 10, 页码 4800-4806出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b13782
关键词
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资金
- National Natural Science Foundation of China [21735004, 21874089, 21927806, 21435004, 21705024, 31800796]
- Innovative research team of high-level local universities in Shanghai
- Program for Changjiang Scholars and Innovative Research Team in University [IRT13036]
The ubiquitous biomembrane interface, with its dynamic lateral fluidity, allows membrane-bound components to rearrange and localize for high-affinity multivalent ligand-receptor interactions in diverse life activities. Inspired by this, we herein engineered a fluidic multivalent nanointerface by decorating a microfluidic chip with aptamer-functionalized leukocyte membrane nanovesicles for high-performance isolation of circulating tumor cells (CTCs). This fluidic biomimetic nanointerface with active recruitment-binding afforded significant affinity enhancement by 4 orders of magnitude, exhibiting 7-fold higher capture efficiency compared to a monovalent aptamer functionalized-chip in blood. Meanwhile, this soft nanointerface inherited the biological benefits of a natural biomembrane, minimizing background blood cell adsorption and maintaining excellent CTC viability (97.6%). Using the chip, CTCs were successfully detected in all cancer patient samples tested (17/17), suggesting the high potential of this fluidity-enhanced multivalent binding strategy in clinical applications. We expect this bioengineered interface strategy will lead to the design of innovative biomimetic platforms in the biomedical field by leveraging natural cell-cell interaction with a natural biomaterial.
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