Daily rhythms of phytomelatonin signaling modulate diurnal stomatal closure via regulating reactive oxygen species dynamics in Arabidopsis

Melatonin is a well-studied neurohormone oscillating in a 24-h cycle in vertebrates. Phytomelatonin is widespread in plant kingdom, but it remains elusive whether this newly characterized putative hormone underlies the regulation by daily rhythms. Here, we report phytomelatonin signaling, as reflected by changes in endogenous concentrations of phytomelatonin and expression of genes associated with biosynthesis of phytomelatonin (AtSNAT1, AtCOMT1, and AtASMT) and its receptor (AtPMTR1), shows 24-h oscillations in Arabidopsis. The variation of reactive oxygen species (ROS) production and scavenging and expression of ROS-related genes significantly decrease in pmtr1 and snat and increase in PMTR1-OE seedlings, indicating the rhythmicity in phytomelatonin signaling is required for maintenance of ROS dynamics. Additionally, the ROS signaling feedback influences the expression of AtSNAT1, AtCOMT1, AtASMT, and AtPMTR1, suggesting the phytomelatonin and ROS signaling are coordinately interrelated. The pmtr1 mutant plants lose diurnal stomatal closure, with stomata remaining open during daytime as well as nighttime and mutants showing more water loss and drought sensitivity when compared with the wild-type Col-0 plants. Taken together, our results suggest that PMTR1-regulated ROS signaling peaks in the afternoon and may transmit the darkness signals to trigger stomatal closure, which might be essential for high water-use efficiency and drought tolerance.