期刊
JOURNAL OF PHYSICS D-APPLIED PHYSICS
卷 53, 期 16, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1361-6463/ab6b95
关键词
phototoxicity; photodamage; super-resolution microscopy; fluorescence
资金
- UK Biotechnology and Biological Sciences Research Council [BB/R000697/1, BB/R021805/1, BB/S507532/1]
- UK Medical Research Council [MR/K015826/1]
- Wellcome Trust [203276/Z/16/Z]
- 4-year MRC Research Studentship
- 4-year BBSRC Research Studentship
- BBSRC [BB/R021805/1, BB/R000697/1] Funding Source: UKRI
- MRC [MR/K015826/1] Funding Source: UKRI
Super-resolution microscopy (SRM) enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we give an overview of new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.
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