期刊
JOURNAL OF PHYCOLOGY
卷 56, 期 2, 页码 404-424出版社
WILEY
DOI: 10.1111/jpy.12966
关键词
diarrhetic shellfish poisoning; dinoflagellate; Dinophysis; dinophysistoxin; environmental clones; harmful algal bloom; okadaic acid; phylogeny
资金
- Assembling the Tree of Life grant (NSF) [EF-0629624]
- NOAA National Centers for Coastal Ocean Science Ecology and Oceanography of Harmful Algal Blooms Program (ECOHAB) [NA17NOS4780184]
- NOAA SeaGrant Aquaculture Research Program [NA14OAR4170093]
- Texas SeaGrant [NA14OAR4170102]
- Delaware Department of Natural Resources and Environmental Control
- Virginia Department of Environmental Quality recurring fund
- Virginia Department of Health recurring fund
Due to the increasing prevalence of Dinophysis spp. and their toxins on every US coast in recent years, the need to identify and monitor for problematic Dinophysis populations has become apparent. Here, we present morphological analyses, using light and scanning electron microscopy, and rDNA sequence analysis, using a 2-kb sequence of ribosomal ITS1, 5.8S, ITS2, and LSU DNA, of Dinophysis collected in mid-Atlantic estuarine and coastal waters from Virginia to New Jersey to better characterize local populations. In addition, we analyzed for diarrhetic shellfish poisoning (DSP) toxins in water and shellfish samples collected during blooms using liquid-chromatography tandem mass spectrometry and an in vitro protein phosphatase inhibition assay and compared this data to a toxin profile generated from a mid-Atlantic Dinophysis culture. Three distinct morphospecies were documented in mid-Atlantic surface waters: D. acuminata, D. norvegica, and a small Dinophysis sp. that was morphologically distinct based on multivariate analysis of morphometric data but was genetically consistent with D. acuminata. While mid-Atlantic D. acuminata could not be distinguished from the other species in the D. acuminata-complex (D. ovum from the Gulf of Mexico and D. sacculus from the western Mediterranean Sea) using the molecular markers chosen, it could be distinguished based on morphometrics. Okadaic acid, dinophysistoxin 1, and pectenotoxin 2 were found in filtered water and shellfish samples during Dinophysis blooms in the mid-Atlantic region, as well as in a locally isolated D. acuminata culture. However, DSP toxins exceeded regulatory guidance concentrations only a few times during the study period and only in noncommercial shellfish samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据