4.6 Article

Photo-immobilization of proteins on carbons

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ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2019.111675

关键词

Bovine serum albumin; alpha-amylase; Photo-immobilization; Carbons; Graphite; Graphene oxide; Graphene oxide modified with SO2

资金

  1. Brazilian Government Agency Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
  2. Spanish Ministerio de Educacion, Cultura y Deporte, DGU [PHB2009-0057-PC]
  3. regional government Xunta de Galicia [ED431B 2017/59]

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The photofunctionalization of three different carbons with two proteins was studied at room temperature. Water solutions of bovine serum albumin, BSA, and alpha-amylase, AA, were photolyzed at 21 degrees C in the presence of graphite microparticles (6.20 mu m), MPG, graphene oxide, MPGO, and graphene oxide modified with SO2, mMPGO. The insertion of BSA on carbon matrixes occurred with a deoxygenation reaction, most likely due to a dehydration step of a water molecule. XPS, TOC and TGA, showed that the BSA photo-insertion on MPG was highly efficient with 34.9% of the weight of MPG after photolysis, with an initial concentration of 1 g.L-1 of BSA. A high yield of AA photoinsertion on the carbons was also obtained. The calculated weight of AA inserted on MPG and MPGO after photolysis was 22.30% and 18.08%, respectively, with respect to the initial weight of carbon, when the initial concentration of AA was 60 mg.L-1. AA immobilized on MPG was active while the enzyme on MPGO showed a smaller activity, within the experimental error. Although a certain extent of denaturalization of both proteins was observed during photolysis, the molecular weight and composition changed very little during the photolysis, which would produce mainly conformational changes and isomerization reactions.

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