期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 201, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2019.111637
关键词
Caenorhabditis elegans; Diplocyclos palmatus; Serratia marcescens; Quorum sensing; UV-induced photoaging
资金
- Department of Science and Technology Promotion of University Research & Scientific Excellence (DST-PURSE)
- Rashtriya Uchchatar Shiksha Abhiyan (RUSA)-Phase 2.0, Government of India [24-51/2014-U]
- Department of Biotechnology (DBT, Goverment of India) [BT/BI/25/012/2012]
- Department of Science and Technology - Fund for Improvement of S&T Infrastructure in Universities and Higher Educational Institutions (DST-FIST, Goverment of India) [SR/FST/LSI-639/2015(C)]
- University Grants Commission - Special Assistance Programme (UGC-SAP, Goverment of India) [F.5-1/2018/DRS-II]
- DST-PURSE (Goverment of India) [SR/PURSE Phase 2/38 (G)]
Plants are considered to be a leading source for possible human therapeutic agents. This holistic study has investigated the anti-quorum sensing (anti-QS), anti-infection, antioxidant and anti-photoaging properties of neglected plant Diplocyclos palmatus. The results showed that D. palmatus methanolic leaf extract (DPME) effectively inhibited the quorum sensing (QS) regulated virulence factor production as well as biofilm formation in Serratia marcescens. The transcriptomic analysis revealed that DPME significantly downed the expression of QS-regulated genes such as fimA, fimC, flhC, bsmB, pigP and shlA in S. marcescens, which supports the outcome of in vitro bioassays. Further, the docking study revealed that the presence of active compounds, namely tocopherols and phytol, DPME exhibited its anti-QS activity against S. marcescens. In addition, DPME treatment extended the lifespan of S. marcescens infected C. elegans by the action of dropping the internal accumulation. Further, qPCR analysis clearly revealed that DPME treatment significantly up-regulated the expression of the lifespan-related gene (daf-16) and immune-related genes (clec-60, clec-87, lys-7 and bec-1) in S. marcescens infected C.elegans. On the other hand, DPME extensively reduced the UV-A induced ROS stress, thereby, extended the lifespan in UV-A photoaged C. elegans. Further, the qPCR analysis also confirmed the up-regulation of daf-16, clec-60, clec-87 and col-19 genes which advocated the improvement of the lifespan, healthspan and collagen production in UV-A photoaged C. elegans. Further bioassays evidenced that that the lifespan extension of photoaged C. elegans was accomplished by the actions of antioxidants such as tocopherols and phytol in DPME.
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