期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 179, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2019.113014
关键词
MALD1-MS imaging; Flavones; Spatial location; Biosynthesis; Metabolic network; Scutellaria baicalensis Georgi
资金
- National Key R&D Program of China [2017YFC1700703, 2017YFC1702701]
- National Natural Science Foundation of China [81891014, 81803662]
Imaging the spatial distributions and dynamics of flavones in heterogeneous plant tissues is significant for our understanding of plant metabolism. Here, we proposed a spatially-resolved approach to map the locations and biosynthesis of flavones in S. baicalensis. A total of 11 flavones, 5 flavone glycosides, 6 carbohydrates, and a variety of flavone synthesis-related metabolites were imaged. Most of these flavone-related metabolites presented stronger ion intensities in root phloem. The biosynthetic network of flavones and their glycosides in S. baicalensis were visualized for the first time. Moreover, we characterized the region-specific activities of four crucial enzymes in flavone synthesis pathway, including L-phenylalanine ammonia-lyase, cinnamate 4-hydroxylase, 4-coumarate coenzyme A ligase, and flavone synthase. In line with the spatial characteristic of flavones, all these four enzymes exhibit higher activity in the root phloem of S. baicalensis. The combination of spatially-resolved metabolites and enzymes information greatly broadens our understanding of flavone biosynthetic network. (C) 2019 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据