期刊
JOURNAL OF ORGANIC CHEMISTRY
卷 85, 期 3, 页码 1476-1483出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.joc.9b02490
关键词
-
资金
- European Research Council (ERC) [678623, 839088]
- European Research Council (ERC) [839088] Funding Source: European Research Council (ERC)
Protein macrocyclization represents a very efficient strategy to increase the stability of protein tertiary structures. Here, we describe a panel of novel C3-symmetric tris-electrophilic agents and their use for the cyclization of proteins. These electrophiles are reacted with a protein domain harboring three solvent-exposed cysteine residues, resulting in the in situ cyclization of the protein (INCYPRO). We observe a clear dependency of cross-linking rates on the electrophilicity. All nine obtained cross-linked protein versions show considerably increased thermal stability (up to 29 degrees C increased melting temperature) when compared to that of the linear precursor. Most interestingly, the degree of stabilization correlates with the hydrophilicity of the cross-link. These results will support the development of novel cross-linked proteins and enable a more rational design process.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据