Anaphylaxis Induced by Peptide Coupling Agents: Lessons Learned from Repeated Exposure to HATU, HBTU, and HCTU

Peptide coupling agents pose a special hazard because they are immune sensitizers. Here, we present a case study of anaphylaxis induced by three uronium coupling agents, HATU, HBTU, and HCTU, as a cautionary note for researchers who handle peptide coupling agents frequently. We also include recommendations for handling coupling agents more safely in the research laboratory.