期刊
JOURNAL OF MOLECULAR LIQUIDS
卷 300, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.molliq.2019.112238
关键词
Unfolding of protein; Refolding of protein; Gemini surfactants; beta-CD/SDS as stripping agents; Spacer chain effect; Nanotube/catanion formation
资金
- UGC-DAE Consortium for Scientific Research, Mumbai Centre (UGC-DAE CSR) [UDCSR/MUM/AO/CRS-M-219/2016/720, UDCSR/MUM/AO/CRS-M-219/2017/975, UDCSR/MUM/AO/CRS-M-219/2017/505]
- Council of Scientific and Industrial Research (CSIR), India [01 (2839)/16/EMR-II]
- University Grants Commission (UGC) [F.540/14/DRS/2007 (SAP-I)]
- UGC-BSR
- Birla Institute of Technology & Science, Pilani (BITS Pilani)
- UGC-DAE CSR
- CSIR
Interactions between protein, BSA and gemini surfactants, 12-n-12 (n = 3, 6, 8,12) and step-by-step refolding of protein present as protein-gemini surfactant complex usingg-cyclodextrin (beta-CD)/sodium dodecyl sulfate (SDS) as stripping agents have been demonstrated by means of UV absorption, steady-state and time-resolved intrinsic fluorescence of BSA, circular dichroism spectroscopy, dynamic light scattering and field emitting scanning electron microscopy. This method is in contrast with the refolding of protein via artificial chaperone protocol. Mechanisms of interactions between protein-gemini complex and beta-CD/SDS have been described. Role of spacer chain of gemini surfactants on the refolding of BSA unfolded by the surfactants has been explained. It has been observed that initially a gemini surfactant molecule with a long flexible spacer chain can more easily be stripped off by beta-CD molecules forming simple inclusion complexes or nanotubes/rods depending on the concentration of beta-CD. After the protein-micelles aggregates are dissociated, refolding of protein occurs more easily in case of gemini surfactant molecules with a short spacer chain. Method of beta-CD induced refolding of a denatured protein has been validated by demonstrating refolding process in case of another protein, lysozyme. Unfolded proteins are also get refolded by SDS through the formation of catanions (mixed micelles, vesicles etc.). (C) 2019 Elsevier B.V. All rights reserved.
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