期刊
JOURNAL OF IMMUNOLOGY
卷 204, 期 5, 页码 1146-1157出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1901170
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资金
- National Institutes of Health (NIH) Research Supplement to Promote Diversity in Health Related Research Grant [AI 105813S-1]
- NIH [UL1 TR001120, T32 AI 138944, AI 079705, AI 105813, AI 124172, AI 131034, AI 135599]
- NIH/National Cancer Institute [P30 CA054174-20]
Upon activation by CD40 or TLR signaling, B lymphocytes activate NF-kappa B to induce activation-induced cytidine deaminase and, therefore, Ig class switch DNA recombination, as central to the maturation of the Ab and autoantibody responses. In this study, we show that NF-kappa B activation is boosted by colocalization of engaged immune receptors, such as CD40, with RAB7 small GTPase on mature endosomes, in addition to signals emanating from the receptors localized on the plasma membrane, in mouse B cells. In mature endosomes, RAB7 directly interacts with TRAF6 E3 ubiquitin ligase, which catalyzes K63 polyubiquitination for NF-kappa B activation. RAB7 overexpression in Cd19(+/cre) Rosa26(fl-STOP-fl-Rab7) mouse B cells upregulates K63 polyubiquitination activity of TRAF6, enhances NF-kappa B activation and activation-induced cytidine deaminase induction, and boosts IgG Ab and autoantibody levels. This, together with the extensive intracellular localization of CD40 and the strong correlation of RAB7 expression with NF-kappa B activation in mouse lupus B cells, shows that RAB7 is an integral component of the B cell NF-kappa B activation machinery, likely through interaction with TRAF6 for the assembly of intracellular membrane signalosomes.
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