Optimizing the production of short and medium chain fatty acids (SCFAs and MCFAs) from waste activated sludge using different alkyl polyglucose surfactants, through bacterial metabolic analysis

Alkyl polyglucose is an environmentally-friendly biosurfactant, which is able to enhance short-chain fatty acids production with different carbon chain lengths and concentrations, during sludge anaerobic fermentation. This presents a promising strategy for sludge re-utilization by effectively converting hazardous sludge into value-added compounds. The maximum yield of short-chain fatty acids produced from sludge was 479.3 and 462.2 mg COD/g VSS, following pretreatment with APG06 and APG1214, respectively. To the best of our knowledge, the short-chain fatty acid production performance by sludge fermentation reported here, achieved a higher level than reported in previous studies. Additionally, these findings indicate that the production of medium-chain fatty acids from sludge can be induced by alkyl polyglucoses. Finally, the microbial community and enzyme activity were also assessed to reveal the mechanism of short-/medium-chain fatty acids biosynthesis under alkyl polyglucose pretreatment. This study demonstrates that alkyl polyglucose provides an environmentally-friendly and effective strategy for enhancing the production of short-/medium-chain fatty acids from waste activated sludge. These findings are useful for the assessment of alkyl polyglucose-assisted production of short-/medium-chain fatty acids, as well as for understanding the interactions between short-/medium-chain fatty acids and microbial communities with key enzymes, to establish short-/medium-chain fatty acids metabolic pathways during sludge fermentation.