Nitrite accumulation in comammox-dominated nitrification-denitrification reactors: Effects of DO concentration and hydroxylamine addition

In this study, nitrite accumulation was investigated under different DO conditions and different hydroxylamine addition methods during the domestic wastewater treatment. Two sequencing batch reactors in parallel were operated under cyclic aerobic and anoxic conditions with the DO concentration of 2.0 and 4.0 mg/L in aerobic phase. The nitrite accumulation rate during high DO conditions increased to 44.8 and 66.7% in 20 days. During hydroxylamine addition, the NAR increased over 90% under the continuous and intermittent hydroxylamine addition. Continuous hydroxylamine addition could result in a more efficient and rapid nitrite accumulation. The findings suggested that comammox could be the main reason for the failure of partial nitrification in a low DO environment ( < 0.5 mg/L). The nitrogen variation during typical cycles showed that the continuous hydroxylamine addition suppressed the activity of NOB and the ammonia oxidation rate. Further, the qPCR results indicated that the abundance of comammox amoA (ranged from 6.25 x 10(7) to 4.16 x 10(8) copies/g VSS) was higher than those of AOB amoA and Nitrobacter in sludge samples. The findings from the current study may enrich our understanding of partial nitrification and its control strategy.