期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 217, 期 3, 页码 -出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20190580
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资金
- Fonds De La Recherche Scientifique [Televie 7.4555.14, J.0078.13]
- Fonds Gaston Ithier
- European Commission (Marie SklodowskaCurie Actions [MSCA] project) [751954]
- Fonds De La Recherche Scientifique (Televie)
- Fondation Rose et Jean Hoguet
- Fonds De La Recherche Scientifique (FRIA)
- Chinese Scholarship Council
- Wellcome Trust
- Fondation Universitaire of Belgium
- Marie Curie Actions (MSCA) [751954] Funding Source: Marie Curie Actions (MSCA)
In the mouse thymus, invariant gamma delta T cells are generated at well-defined times during development and acquire effector functions before exiting the thymus. However, whether such thymic programming and age-dependent generation of invariant gamma delta T cells occur in humans is not known. Here we found that, unlike postnatal gamma delta thymocytes, human fetal gamma delta thymocytes were functionally programmed (e.g., IFN gamma, granzymes) and expressed low levels of terminal deoxynucleotidyl transferase (TdT). This low level of TdT resulted in a low number of N nucleotide insertions in the complementarity-determining region-3 (CDR3) of their TCR repertoire, allowing the usage of short homology repeats within the germline-encoded VDJ segments to generate invariant/public cytomegalovirus-reactive CDR3 sequences (TRGV8-TRJP1-CATWDTTGWFKIF, TRDV2-TRDD3-CACDTGGY, and TRDV1-TRDD3-CALGELGD). Furthermore, both the generation of invariant TCRs and the intrathymic acquisition of effector functions were due to an intrinsic property of fetal hematopoietic stem and precursor cells (HSPCs) caused by high expression of the RNA-binding protein Lin28b. In conclusion, our data indicate that the human fetal thymus generates, in an HSPC/Lin28b-dependent manner, invariant gamma delta T cells with programmed effector functions.
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