期刊
JOURNAL OF ENDODONTICS
卷 46, 期 1, 页码 65-73出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2019.10.001
关键词
Apical periodontitis; inducible nitric oxide synthase; lipopolysaccharide; metformin; monocyte
资金
- Ministry of Science and Technology of Taiwan [MOST103-2314-B-002-094-MY2, MOST 105-2314-B-002-076, MOST 106-2314-B-002-026-MY3, MOST104-2314-13-002-154-MY3]
- National Taiwan University Hospital [NTUH105-S3016, NTUH106-S3422, NTUH107-S3765, NTUH108-S4149, NTUH105-53076, NTUH106-S3489]
Introduction: We have previously shown that intracanal metformin ameliorates apical periodontitis, partially by modulation of osteoblast apoptosis. The action of metformin on other cell types pertinent to the development of apical periodontitis needs to be examined. In the present study, we aimed to analyze whether its effects on the expression of inducible nitric oxide synthase (iNOS) and monocyte recruitment contribute to the therapeutic effect on apical periodontitis. Methods: Lipopolysaccharide (LPS)-induced expression of iNOS in a human monocytic cell line, Mono-Mac-6, was assessed by Western blot. The amount of nitrite in culture medium was assessed to quantify nitric oxide (NO) production. C-C motif chemokine ligand-2 (CCL-2) synthesis was measured by enzyme-linked immunosorbent assay. Experimental apical periodontitis in rats was treated with root canal debridement with or without intracanal metformin medication. Lesion progression was assessed by conventional radiography and micro-computed tomographic imaging. Cellular expression of iNOS and the number of monocytes/macrophages were assessed by immunohistochemistry. Results: Metformin suppressed LPS-induced iNOS and NO production by monocytes. More importantly, metformin inhibited LPS-enhanced CCL-2 synthesis through modulation of the iNOS/NO pathway. Intracanal metformin reduced bone resorption associated with apical periodontitis and suppressed iNOS expression and monocyte recruitment. Conclusions: Our results confirmed the therapeutic efficacy of intracanal metformin for apical periodontitis. Suppression of monocyte recruitment through modulation of iNOS expression and NO production is an important mechanism underlying the beneficial effect of metformin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据