A novel disposable electrochemical sensor based on modifying graphite pencil lead electrode surface with nanoacetylene black for simultaneous determination of antiandrogens flutamide and cyproterone acetate

A novel nanoacetylene black coated the surface of graphite pencil lead electrode (nano-AB/GPLE) is successfully constructed. Electrochemical impedance spectroscopy (EIS) and cyclic voltarnmetry (CV) indicated that the new designed nano-AB/GPLE possessed a fast electron transfer compared with the bare GPLE and enhanced electrochemical response toward electroreduction of antiandrogens flutamide (FLU) and cyproterone acetate (CPA) simultaneously. From the nano-AB/GPLE, two well separated reduction signals of coexistence FLU and CPA at nano-AB/GPLE were obtained. Electrochemical sensing of FLU and CPA in the presence of the physiological common interferents at nano-AB/GPLE was examined and confirmed the selectivity for the detection analysis of the two antiandrogens without cross interference of biological compounds. The limits of detection for coexistence of FLU and CPA are 1.39 nM and 4.74 nM, respectively. Finally, the applicability of the designed sensor is performed for detection of the two antiandrogens FLU and CPA simultaneously in pharmaceutical tablets and biological fluids with good accuracy and precision. (C) 2020 Elsevier B.V. All rights reserved.