4.6 Article

Modeling the nonlinear behavior of a bioactive peptide in reversed-phase gradient elution chromatography

期刊

JOURNAL OF CHROMATOGRAPHY A
卷 1616, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.chroma.2019.460789

关键词

Pharmaceutical peptides; Gradient elution; Adsorption isotherms; Reversed-phase liquid chromatography (RP-LC); Inverse method; Preparative chromatography

资金

  1. Italian University and Scientific Research Ministry [PRIN 2017Y2PAB8_003]

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The thermodynamic behavior of octreotide, a cyclic octapeptide with important pharmaceutical functions, has been simulated under reversed-phase gradient elution conditions. To this end, adsorption behavior was firstly investigated in isocratic conditions, under a variety of water/acetonitrile + 0.02% (v/v) trifluoroacetic acid (TFA) mixtures as mobile phase by using a Langmuir isotherm. Organic modifier was varied in the range between 23 and 28% (v/v). Adsorption isotherms were determined by means of the so-called Inverse Method (IM) with a minimum amount of peptide. The linear solvent strength (LSS) model was used to find the correlation between isotherm parameters and mobile phase composition. This study contributes to enlarge our knowledge on the chromatographic behavior under nonlinear gradient conditions of peptides. In particular, it focuses on a cyclic octapeptide. (C) 2019 Elsevier B.V. All rights reserved.

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