期刊
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
卷 95, 期 5, 页码 1519-1532出版社
WILEY
DOI: 10.1002/jctb.6345
关键词
biodegradable; downstream; environmental biotechnology; purification; waste treatment and waste minimization; bioprocesses
类别
资金
- European Commission [613667]
- Technical University of Denmark
BACKGROUND Purification of polyhydroxyalkanoates (PHA) is a challenging step, given the difficulty of achieving high PHA purity, while maintaining polymer integrity, in a sustainable and cost-efficient manner. This study evaluated the potential of dilute ammonia digestion as a method to purify PHA from mixed microbial consortia. RESULTS Digestion temperatures were critical to the obtainable purity and the amount of recovered PHA. At temperatures below 75 degrees C (regardless of the incubation time and ammonia concentration), a low PHA recovery (down to 65%) and no increase in purity was observed. By increasing the temperature above 75 degrees C, a significantly higher PHA purity and higher recovery (above 90%) could be achieved. Temperatures maximizing the purity (140 degrees C) led to a detrimental reduction in the molar mass of the isolated PHA, but the use of a sonication pre-treatment enabled to increase the purity at temperatures leading to limited molar mass loss (75-115 degrees C). The impurities still present in the recovered PHA did not compromise its thermal stability, and no significant degradation occurred during melting of PHA with 86% purity (comparably to pure chloroform-extracted PHA). Conversely, PHA recovered through sulphuric acid (H2SO4) digestion underwent severe degradation during melting, despite presenting higher purity (98%). CONCLUSIONS High PHA purity, recovery and thermal stability can be obtained with dilute ammonia digestion. These observations, combined with the possibility of reusing ammonia within the process, make this method a promising approach for a more sustainable purification of PHA. (c) 2020 Society of Chemical Industry
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据